Complement Factor H antibody

Catalog No. ABIN462334

This Mouse Monoclonal antibody specifically detects Complement Factor H in WB, IHC, IP, ELISA and IHC (p). It exhibits reactivity toward Human.

Quick Overview for Complement Factor H antibody (ABIN462334)

Target: Complement Factor H (CFH)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Complement Factor H antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: OX-24

Product Details anti-Complement Factor H Antibody

Brand: IHC-plus™

Specificity: Recognizes the human serum complement protein factor H (155kD), binding to a different epitope from MRC OX23. The Recognizes a 43-49kD truncated form of factor H present at low level (1-5 μg/mL) in plasma and urine. In an immunoprecipitation system. Recognizes factor H in other primates, but not in cow, sheep, pig, chicken or rabbit sera. Inhibits the binding of factor H to surface bound C3b, Recognizes Factor H in Western blotting procedures.

Purification: Protein G purified

Immunogen: Human complement factor H.

Type of Immunogen: Purified protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (10 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Not recommended for: Flo

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Complement Factor H

Target: Complement Factor H (CFH)

Alternative Name: CFH / Complement Factor H

Background: Name/Gene ID: CFH

Synonyms: CFH, Adrenomedullin binding protein, AHUS1, AMBP1, ARMS1, ARMD4, Beta-1-H-globulin, Factor H, Factor H-like 1, HF, HF2, H factor 1, H factor 2 (complement), HUS, HF1, Beta-1H, CFHL3, Complement factor H, H factor 1 (complement)

Gene ID: 3075

UniProt: P08603

Pathways: Complement System, Cellular Response to Molecule of Bacterial Origin